Virus isolation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) for diagnostic and research purposes

  • Sacha Stelzer-Braid
    Correspondence
    Address for correspondence: Dr Sacha Stelzer-Braid, Virology Research Laboratory, Level 3 Clinical Sciences Building, Prince of Wales Hospital, Randwick, NSW, 2031. Australia.
    Affiliations
    Virology Research Laboratory, Serology and Virology Division (SAViD), Prince of Wales Hospital, Sydney, NSW, Australia

    School of Medical Sciences, Faculty of Medicine, University of New South Wales, Sydney, NSW, Australia
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  • Gregory J. Walker
    Affiliations
    Virology Research Laboratory, Serology and Virology Division (SAViD), Prince of Wales Hospital, Sydney, NSW, Australia

    School of Medical Sciences, Faculty of Medicine, University of New South Wales, Sydney, NSW, Australia
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  • Anupriya Aggarwal
    Affiliations
    Kirby Institute, University of New South Wales, Sydney, NSW, Australia
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  • Sonia R. Isaacs
    Affiliations
    Virology Research Laboratory, Serology and Virology Division (SAViD), Prince of Wales Hospital, Sydney, NSW, Australia

    School of Medical Sciences, Faculty of Medicine, University of New South Wales, Sydney, NSW, Australia
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  • Malinna Yeang
    Affiliations
    Virology Research Laboratory, Serology and Virology Division (SAViD), Prince of Wales Hospital, Sydney, NSW, Australia

    Serology and Virology Division (SAViD), NSW Health Pathology East, Department of Microbiology, Prince of Wales Hospital, Sydney, NSW, Australia
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  • Zin Naing
    Affiliations
    Serology and Virology Division (SAViD), NSW Health Pathology East, Department of Microbiology, Prince of Wales Hospital, Sydney, NSW, Australia
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  • Alberto Ospina Stella
    Affiliations
    Kirby Institute, University of New South Wales, Sydney, NSW, Australia
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  • Stuart G. Turville
    Affiliations
    Kirby Institute, University of New South Wales, Sydney, NSW, Australia
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  • William D. Rawlinson
    Affiliations
    Virology Research Laboratory, Serology and Virology Division (SAViD), Prince of Wales Hospital, Sydney, NSW, Australia

    School of Medical Sciences, Faculty of Medicine, University of New South Wales, Sydney, NSW, Australia

    Serology and Virology Division (SAViD), NSW Health Pathology East, Department of Microbiology, Prince of Wales Hospital, Sydney, NSW, Australia
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Published:October 08, 2020DOI:https://doi.org/10.1016/j.pathol.2020.09.012

      Summary

      Isolation of the new pandemic virus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is essential for diagnostic and research purposes including assessment of novel therapeutics. Several primary and continuous cell lines are currently used, and new organoid and engineered cell lines are being developed for improved investigation and understanding of the human immune response to this virus. Here we review the growth of SARS-CoV-2 in reference standard cell lines, engineered cell lines and new developments in this field.

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