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Research Article| Volume 44, ISSUE 6, P557-561, October 2012

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Altered IkBα expression promotes NF-kB activation in monocytes from primary Sjögren’s syndrome patients

  • Author Footnotes
    § these authors contributed equally and should be considered as first authors.
    Sabrina Lisi
    Correspondence
    Address for correspondence: Department of Basic Medical Sciences, Section of Human Anatomy, University of Bari, Piazza Giulio Cesare 1, I-70124 Bari, Italy
    Footnotes
    § these authors contributed equally and should be considered as first authors.
    Affiliations
    Department of Basic Medical Sciences, Section of Human Anatomy, Laboratory of Cell Biology, Italy
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  • Author Footnotes
    § these authors contributed equally and should be considered as first authors.
    Margherita Sisto
    Footnotes
    § these authors contributed equally and should be considered as first authors.
    Affiliations
    Department of Basic Medical Sciences, Section of Human Anatomy, Laboratory of Cell Biology, Italy
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  • Dario Domenico Lofrumento
    Affiliations
    Department of Biological and Environmental Sciences and Technologies, University of Salento, Lecce, Italy
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  • Massimo D’Amore
    Affiliations
    Department of Internal Medicine and Public Medicine, Section of Rheumatology, University of Bari Medical School, Bari, Italy
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  • Author Footnotes
    § these authors contributed equally and should be considered as first authors.
DOI:https://doi.org/10.1097/PAT.0b013e3283580388
Altered IkBα expression promotes NF-kB activation in monocytes from primary Sjögren’s syndrome patients
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      Summary

      Aims

      To study the importance of IkBα in NF-kB signal transduction, we analysed the IkBα expression in monocytes from Sjögren’s syndrome (SS) patients versus healthy controls.

      Methods

      Monocytes were obtained from the peripheral blood of 30 SS patients and 23 healthysubjects. I kBa expression was studied by semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR), real-time PCR, immunoblotting, flow cytometry and enzyme linked immunosorbent assay (ELISA).

      Results

      Analysis of the gene and protein expression profiles of SS monocytes revealed a down-regulation of IkBα, and in all the Sjögren’s syndrome cases examined, serum IkBa levels were significantly decreased in comparison with controls.

      Conclusions

      Our findings clearly demonstrate changes in the levels of IkBα in SS monocytes, suggesting that the attenuated expression of IkBα could contribute to the deregulation of NF-kB pathways in the SS pathogenesis. Decreased expression of IkBα may specifically amplify cytokines production and inflammatory response linked to Sjögren’s syndrome.

      Key words

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      Article info

      Publication history

      Accepted: February 21, 2012
      Received in revised form: February 14, 2012
      Received: January 10, 2012

      Identification

      DOI: https://doi.org/10.1097/PAT.0b013e3283580388

      Copyright

      © 2012 Royal College of Pathologists of Australasia. Published by Elsevier Inc. All rights reserved.

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