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Research Article| Volume 47, ISSUE 3, P191-198, April 2015

Rapid identification of pathogens using molecular techniques

  • Theo P. Sloots
    Affiliations
    Queensland Paediatric Infectious Diseases Laboratory, Queensland Children’s Medical Research Institute, Children’s Health Queensland, Brisbane, Australia

    Microbiology Department, Pathology Queensland, Brisbane, Qld, Australia
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  • Michael D. Nissen
    Affiliations
    Queensland Paediatric Infectious Diseases Laboratory, Queensland Children’s Medical Research Institute, Children’s Health Queensland, Brisbane, Australia

    Microbiology Department, Pathology Queensland, Brisbane, Qld, Australia
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  • Andrew N. Ginn
    Affiliations
    Centre for Infectious Diseases and Microbiology, University of Sydney, Westmead Hospital, Westmead, Australia

    Centre for Research Excellence in Critical Infection, Marie Bashir Institute for Infectious Diseases and Biosecurity and Westmead Millennium Institute, University of Sydney, Sydney, NSW, Australia
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  • Jonathan R. Iredell
    Correspondence
    Address for correspondence: Centre for Infectious Diseases and Microbiology, University of Sydney, Westmead Hospital, Westmead, NSW 2145, Australia.
    Affiliations
    Centre for Infectious Diseases and Microbiology, University of Sydney, Westmead Hospital, Westmead, Australia

    Centre for Research Excellence in Critical Infection, Marie Bashir Institute for Infectious Diseases and Biosecurity and Westmead Millennium Institute, University of Sydney, Sydney, NSW, Australia
    Search for articles by this author
DOI:https://doi.org/10.1097/PAT.0000000000000241
Rapid identification of pathogens using molecular techniques
Previous ArticleContemporary molecular approaches in the clinical microbiology laboratory: progress and pitfalls
Next ArticleWhole genome sequencing in clinical and public health microbiology
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      Summary

      Real-time PCR is the traditional face of nucleic acid detection in the diagnostic microbiology laboratory and is now generally regarded as robust enough to be widely adopted. Methods based on nucleic acid detection of this type are bringing increased accuracy to diagnosis in areas where culture is difficult and/or expensive, and these methods are often effective partners to other rapid molecular diagnostic tools such as matrix-assisted laser desorption ionisation-time of flight mass spectrometry (MALDI-TOF MS). This change in practice has particularly affected the recognition of viruses and fastidious or antibiotic-exposed bacteria, but has been also shown to be effective in the recognition of troublesome or specialised phenotypes such as antiviral resistance and transmissible antibiotic resistance in the Enterobacteriaceae. Quantitation and high-intensity sequencing (of multiple whole genomes) has brought new opportunities as well as new challenges to the microbiology community. Diagnostic microbiologists currently training might be expected to deal less with the culturebased techniques of the last half-century than with the high-volume data and complex analyses of the next.

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      Article info

      Publication history

      Accepted: February 5, 2015
      Received in revised form: February 4, 2015
      Received: December 8, 2014

      Identification

      DOI: https://doi.org/10.1097/PAT.0000000000000241

      Copyright

      © 2015 Royal College of Pathologists of Australasia. Published by Elsevier Inc. All rights reserved.

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